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Application of the mini-Mu phage for the isolation of lac transcriptional fusions in Bacillus subtilis genes

✍ Scribed by Gardiol, Daniela; Gramajo, Hugo C.; Hirschbein, Luisa; Mendoza, Diego de

Book ID
122074178
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
692 KB
Volume
123
Category
Article
ISSN
0378-1119

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A gene coding for a modifying DNA-methyltransferase which methylates the central C in the BsuR recognition sequence 5'GGCC was identified in the genome of the temperate Bacillus subtilis phage SPfi. This gene is expressed only after induction of the prophage by either mitomycin C or UV. The presence