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Application of the iodine–azide postcolumn reaction in RP-HPLC for the determination of thioguanine in urine

✍ Scribed by Robert Zakrzewski

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
473 KB
Volume
31
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

The study focuses on the analysis of a sensitive, selective, and simple postcolumn detection method for thioguanine determination, based on the sensitizing induction of thioguanine on iodine–azide reaction and the combination technique of HPLC. The analysis was accomplished in the optimum conditions for iodine–azide detection system and HPLC separation. The values for the linear range, the LOD, and DOQ amounted to 0.8–1.7, 0.4, and 0.5 nmol/mL urine, respectively.

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