We have established a sensitive ELISPOT assay measuring interferon g (IFN g) release on a single-cell basis to detect influenza peptide-specific CD8 1 T cells in uncultured peripheral blood mononuclear cells (PBMC). Using this method, we studied the T cell response to HLA-A1 and HLA-A2.1 binding pep
✦ LIBER ✦
Application of the IFN-γ ELISPOT assay to quantify T cell responses against proteins
✍ Scribed by Alexander Schmittel; Ulrich Keilholz; Sandra Bauer; Ulrike Kuhne; Stefan Stevanovic; Eckhard Thiel; Carmen Scheibenbogen
- Book ID
- 114050653
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 199 KB
- Volume
- 247
- Category
- Article
- ISSN
- 0022-1759
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