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Application of the FLIPSY Pulse Sequence for Increased Sensitivity in 1H NMR-Based Metabolic Profiling Studies

✍ Scribed by Lauridsen, Michael; Maher, Anthony D.; Keun, Hector; Lindon, John C.; Nicholson, Jeremy K.; Nyberg, Nils T.; Hansen, Steen H.; Cornett, Claus; Jaroszewski, Jerzy W.

Book ID
111895060
Publisher
American Chemical Society
Year
2008
Tongue
English
Weight
255 KB
Volume
80
Category
Article
ISSN
0003-2700

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Application of reverse-DEPT polarization
Application of reverse-DEPT polarization transfer pulse sequence to study the metabolism of carbon-13-labeled substrates in perfused organs by 1H NMR Spectroscopy
✍ F. Desmoulin; S. Confort-Gouny; S. Masson; M. Bernard; D. M. Doddrell; P. J. Coz πŸ“‚ Article πŸ“… 1990 πŸ› John Wiley and Sons 🌐 English βš– 344 KB

## Abstract Metabolism of ^13^C‐enriched metabolites can be advantageously studied by reverse‐polarization transfer methods. In this work an improved reverse‐DEPT sequence has been applied for the first time on perfused organs in a 20‐mm probe. The metabolic fate of 99% enriched [ 2‐^13^C] acetate