## Abstract Metabolism of ^13^C‐enriched metabolites can be advantageously studied by reverse‐polarization transfer methods. In this work an improved reverse‐DEPT sequence has been applied for the first time on perfused organs in a 20‐mm probe. The metabolic fate of 99% enriched [ 2‐^13^C] acetate
✦ LIBER ✦
Application of the reverse dept polarization-transfer pulse sequence to monitor in vitro and in vivo metabolism of 13C-ethanol by 1H-NMR spectroscopy
✍ Scribed by Michael G. Irving; Stephen J. Simpson; William M. Brooks; Roger S. Holmes; David M. Doddrell
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 782 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0020-711X
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Alanine is the major amino acid utilized by the liver for gluconeogenesis under normal conditions. The metabolism of alanine in rat liver was investigated by means of 1 H and 13 C NMR spectroscopic studies in vivo and in vitro after infusion of L-and D-alanine labelled with 13 C at the carboxyl and