𝔖 Bobbio Scriptorium
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Application of temperature-induced phase partitioning at ambient temperature for enzyme purification

✍ Scribed by Patricia A. Alred; Antoni Kozlowski; J.Milton Harris; Folke Tjerneld

Book ID
107785468
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
917 KB
Volume
659
Category
Article
ISSN
1873-3778

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✍ Alvaro Sánchez-Ferrer; Roque Bru; Francisco García-Carmona 📂 Article 📅 1990 🏛 Elsevier Science 🌐 English ⚖ 444 KB

Triton X-114 was used to partially purify broad bean polyphenol oxidase, a thylakoid membrane-bound enzyme, in latent form, free of phenolic compounds and chlorophylls, with a high recovery rate. The activation of the latent enzyme by detergents or trypsin was 10 times higher than that obtained when