𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Application of NMR screening techniques for observing ligand binding with a protein receptor

✍ Scribed by Sakurako Shimotakahara; Kazuo Furihata; Mitsuru Tashiro

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
116 KB
Volume
43
Category
Article
ISSN
0749-1581

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Water ligand observed via gradient spectroscopy (WaterLOGSY), saturation transfer difference and NOE pumping NMR techniques were used to identify ligand binding with a receptor. Although these experiments were originally designed to observe ligands in complexes, their application is limited by the affinity of ligands towards target molecules. Here the improved WaterLOGSY pulse sequence was developed by incorporating the double pulsed field gradient spin‐echo and gradient‐tailored excitation WATERGATE sequences. The efficiency of these ligand‐observed NMR screening techniques was investigated using the ribonuclease T~1~‐inhibitor system. Copyright Β© 2004 John Wiley & Sons, Ltd.

πŸ“œ SIMILAR VOLUMES

An efficient use of the WATERGATE W5 seq
An efficient use of the WATERGATE W5 sequence for observing a ligand binding with a protein receptor
✍ Kazuo Furihata; Sakurako Shimotakahara; Mitsuru Tashiro πŸ“‚ Article πŸ“… 2008 πŸ› John Wiley and Sons 🌐 English βš– 190 KB

## Abstract An efficient pulse sequence for observing a ligand binding with a receptor has been developed by incorporating the WATERGATE W5 sequence. In the conventional water ligand observed via gradient spectroscopy (WaterLOGSY) techniques, the water resonance is selectively excited using,e.g. th