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An efficient use of the WATERGATE W5 sequence for observing a ligand binding with a protein receptor

✍ Scribed by Kazuo Furihata; Sakurako Shimotakahara; Mitsuru Tashiro

Publisher: John Wiley and Sons
Year: 2008
Tongue: English
Weight: 190 KB
Volume: 46
Category: Article
ISSN: 0749-1581
DOI: 10.1002/mrc.2264

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✦ Synopsis

Abstract

An efficient pulse sequence for observing a ligand binding with a receptor has been developed by incorporating the WATERGATE W5 sequence. In the conventional water ligand observed via gradient spectroscopy (WaterLOGSY) techniques, the water resonance is selectively excited using,e.g. the double‐pulsed field gradient spin–echo (DPFGSE) sequence at the initial portion of pulse sequence. In the current version, the modified WATERGATE W5 sequence is incorporated at the initial portion of the pulse sequence, and the resonance at the water frequency can be selectively reserved by the modified WATERGATE W5 sequence. The efficiency of ligand‐observed NMR screening techniques has been demonstrated using the human serum albumin (HSA)–tryptophan complex. Copyright © 2008 John Wiley & Sons, Ltd.

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