Application of NMR in Structural Proteomics: Screening for Proteins Amenable to Structural Analysis
โ Scribed by Till Rehm; Robert Huber; Tad A Holak
- Book ID
- 114329187
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 175 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0969-2126
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A simple and rapid method based on 15N labeling and 1H-15N heteronuclear single quantum coherence spectroscopy is presented to directly assess the structural integrity of overexpressed proteins in crude Escherichia coli extracts without the need for any purification. The method is demonstrated using
A collaborative project between two Structural Proteomics In Europe (SPINE) partner laboratories, York and Oxford, aimed at high-throughput (HTP) structure determination of proteins from __Bacillus anthracis__, the aetiological agent of anthrax and a biomedically important target, is described. Base