๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Application of mass spectrometry for target identification and characterization

โœ Scribed by Joseph A. Loo; Dana E. DeJohn; Ping Du; Tracy I. Stevenson; Rachel R. Ogorzalek Loo

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
175 KB
Volume
19
Category
Article
ISSN
0198-6325

No coin nor oath required. For personal study only.

โœฆ Synopsis

Mass spectrometry-based methodologies span the vast expanse of drug discovery. Both electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) support proteomics-based research projects by identifying proteins separated and isolated by polyacrylamide gel electrophoresis. MALDI-MS-based surface scanning of one-dimensional isoelectric focusing gels, "virtual 2-D gel electrophoresis," represents a potentially high throughput means to map proteins and to determine protein profiles. Mass spectrometry can also be used to directly study the covalent and noncovalent interactions of drug molecules and biomolecule targets. Drug binding examples discussed include the binding of covalent and noncovalent inhibitors to src SH2 domain protein, and the interaction of aminoglycoside antibiotic neomycin and HIV Tat peptide-TAR RNA.

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