Application of isotachophoretic and conductometric methods for neomycin trisulphate determination

✍ Scribed by Marzanna Kurzawa; Aneta Jastrzębska; Edward Szłyk

• Book ID: 111491216
• Publisher: Versita
• Year: 2009
• Tongue: English
• Weight: 260 KB
• Volume: 63
• Category: Article
• ISSN: 0366-6352
• DOI: 10.2478/s11696-008-0103-2

✦ Synopsis

Abstract

Determination of neomycin trisulphate (NMS) in a dosage form (Neox and Neosol) was carried out by capillary isotachophoresis (cITP) with conductometric detection. The following electrolytes: leading: 10 mmol dm−3 sodium acetate + 0.08 % hydroxyethylcelulose (HEC) and acetic acid to pH = 5.5, and terminating: 10 mmol dm−3 β-alanine were tested for isotachophoretic separation of NMS. The calibration curve was linear over the range of 10.00 mg dm−3 to 100.00 mg dm−3 with LOD = 5.69 mg dm−3 and LOQ = 18.96 mg dm−3. The results were compared to the conductometric determination of NMS with: ammonium molybdate (VI), silver nitrate (V) and Reinecke salt. Good accuracy was obtained from conductometric titration of NMS with Reinecke salt, the recoveries being as follows: 100 % (RSD = 1.99 %); 96.17 % (RSD = 2.10 %) and 95.22 % (RSD = 1.55 %) for NMS in pure form, Neosol and Neox, respectively.