Application of high-performance liquid chromatography/electrospray mass spectrometry for the characterization of membrane lipids in the haloalkaliphilic archaebacterium Natronobacterium magadii

✍ Scribed by Di-Fei Qiu; Marguerite P. L. Games; Xiao-Yao Xiao; David E. Games; Terence J. Walton

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 213 KB
• Volume: 12
• Category: Article
• ISSN: 0951-4198
• DOI: 10.1002/(sici)1097-0231(19980731)12:14<939::aid-rcm261>3.0.co;2-f

✦ Synopsis

The membrane lipid composition of Natronobacterium magadii, a species of haloalkaliphilic archaebacterium, has been studied by electrospray mass spectrometry (ES-MS), reversed phase high-performance liquid chromatography/electrospray mass spectrometry (LC/ES-MS), and tandem mass spectrometry. The negative-ion and positive-ion electrospray mass spectra and product ion mass spectra of the polar lipids are reported. LC/ES-MS of the total lipid extract in conjunction with product ion mass spectrometry established diphytanyl-and phytanyl, sesterterpanylglycerol-diether analogues of phosphatidylglycerolphosphate methyl ester as the major archaeols, with much lower levels of diphytanyl-and phytanyl, sesterterpanylglycerol-diether analogues of phosphatidylglycerol. In addition to these saturated, isopranyl archaeols, the presence of unsaturated archaeol analogues containing up to three double bonds was detected in Nb. magadii, representing the first description of isoprenyl ether lipids in a haloalkaliphilic archaebacterium.