Application of high-performance liquid chromatographic separation of free sterols to the screening of yeast sterol mutants

✍ Scribed by R.J. Rodriguez; L.W. Parks

• Book ID: 102629259
• Publisher: Elsevier Science
• Year: 1982
• Tongue: English
• Weight: 410 KB
• Volume: 119
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(82)90686-8

✦ Synopsis

A method for the rapid separation of free sterols by reversed-phase liquid chromatography has been developed. A trinary mobile phase of ethanol, methanol, and water pumped through an ODS (octadecylsilane)~Ultrasphere column allowed for the separation of sterols on the basis of the number and location of double bonds and alkyl groups. This method is utilized to screen yeast mutants for altered sterol patterns.