Application of gas-liquid chromatography to phenol oxidase studies

✍ Scribed by Benjamin C. Garrett; A.L. Underwood

• Publisher: Elsevier Science
• Year: 1977
• Tongue: English
• Weight: 388 KB
• Volume: 78
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(77)90016-1

✦ Synopsis

Extraction with ethyl acetate followed by gas-liquid chromatography on the extract provides a useful analytical method for phenol oxidase studies where it is desired to monitor directly the concentrations of volatile phenolic and catecholic substrates. A packed column with 10% DC-710 on Chromosorb W-AW-DMCS is recommended for such compounds as p-cresol, catechol, and 4-methylcatechol. p-Methoxyphenol is a suitable internal standard to minimize problems in the handling and injection of the extract. Examples of cresolase and catecholase kinetic runs are presented.

1 Variously called tyrosinase, cresolase, phenolase, phenolase complex, catecholase, catechol oxidase, o-diphenol:OZ oxidoreductase, etc.; the most recently recommended systematic name is monophenol-dihydroxyphenylalanine:O, oxidoreductase, EC 1.14.18.1 (1). The recommended trivial name, monophenol monooxygenase (1) is much less used at this time than is phenol oxidase or tyrosinase.