Application of gas-liquid chromatography to analysis of carbon-14 biosynthetically labelled compounds

✍ Scribed by M. Matucha; V. Svoboda; E. Smolková

• Publisher: Elsevier Science
• Year: 1974
• Tongue: English
• Weight: 544 KB
• Volume: 91
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)97931-2

✦ Synopsis

Preparations of higher fatty acids and amino acids of IO-60 mCi/milliatom C specific activity, obtained from algae grown in a 14C02 atmosphere, were analysed by gas-liquid chromatography and radiochemical and chemical purities, ; content and specific activity of isolated compounds and mixtures were determined. Special attention was paid to the distribution of carbon-14 in the individual carbon positions in the fatty acids obtained by common labelling procedures. The results enable the choice of optimal growth conditions for the preparation of uniformly labelled compounds. In addition to mass detection, radioactivity was simultaneously detected by a gas-flow proportional counter, with digital and rate-meter outputs, after combustion on CuO of the split stream.