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Application of competitive ligand-binding radioassays to the nucleotides

โœ Scribed by Alton L. Steiner

Book ID
118947288
Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
512 KB
Volume
22
Category
Article
ISSN
1532-8600

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Competitive binding assays for high-affi
Competitive binding assays for high-affinity binders in the presence of endogenous ligands: Application to biotin-binding proteins
โœ Richard W. Schreiber Jr.; Michael A. Letavic; Timothy J. McGahan; Harold B. Whit ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 731 KB

Endogenous ligands complicate radioligand-binding assays of high-affinity binding proteins by obscuring binding sites or by diluting the labeled ligand. We have developed a mathematical model for such systems where radioligand and endogenous ligand are structurally identical. Data which relate radio