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Application of capillary electrophoresis to the analysis of soluble chromatin

โœ Scribed by Nino V. Asatiani; Marina K. Abuladze; Barbara G. Birkaya; Nelly A. Sapojnikova; Nelly Ya. Tsibakhashvili; Liguri M. Mosulishvili

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
55 KB
Volume
14
Category
Article
ISSN
0269-3879

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โœฆ Synopsis

Capillary electrophoresis (CE) has been applied to study DNA-protein complexes using as the test system soluble chromatin from chicken erythrocytes and rapidly proliferated cultured Chinese hamster fibroblast-like cells B11-dii-FAF-28. Separation was performed with home-made CE apparatus, using a regulated high-voltage power supply, UV-detector and fused silica capillaries with inner diameter 75 mm. The heterogeneity of nucleosomal particles with different DNA lengths after micrococcal nuclease digestion was detected.

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