Application of a coulometric oxygen sensor to the determination of hemoglobin-oxygen binding isotherms
β Scribed by Robert N. Haire; Bo E. Hedlund; Paul A. Hersch
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 488 KB
- Volume
- 78
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
A thin-layer sample cell for examination of the spectra of hemoglobin at various degrees of gaseous-ligand saturation has been constructed to fit into a Cary 17 spectrophotometer. Small sample volumes of 50 ~1 are used. Equilibration times of only a few minutes are required between taking spectra at
## Abstract Analysis of experimental equilibrium constants for the oxygenation of hemoglobin leads to a plausible mechanism for the effect of pH and of chloride ions on cooperativity in hemoglobin. According to this mechanism, the structural changes responsible for cooperativity in chlorideβ and 2,