✦ LIBER ✦

A thin-layer optical cell for spectroscopic studies of oxygen binding to hemoglobin

✍ Scribed by J.D. Mahoney; S.C. Ross; S.J. Gill

Publisher: Elsevier Science
Year: 1977
Tongue: English
Weight: 439 KB
Volume: 78
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(77)90114-2

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✦ Synopsis

A thin-layer sample cell for examination of the spectra of hemoglobin at various degrees of gaseous-ligand saturation has been constructed to fit into a Cary 17 spectrophotometer. Small sample volumes of 50 ~1 are used. Equilibration times of only a few minutes are required between taking spectra at different degrees of saturation. Apparatus stability is high as revealed by reproducibility and isosbestic point behavior. Incorporation of an oxygen electrode enables simultaneous measurement of oxygen partial pressure for intermediate spectra.

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