Applicability of monoclonal antibody Fab fragments as a carrier of neocarzinostatin in targeting chemotherapy

✍ Scribed by Otsuji, Eigo; Yamaguchi, Toshiharu; Tsuruta, Hiroshi; Yata, Yoshihiro; Nishi, Hiroshi; Okamoto, Kazuma; Taniguchi, Katsunori; Kato, Makoto; Kotani, Tatsuya; Kitamura, Kazuya; Takahashi, Toshio

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 519 KB
• Volume: 61
• Category: Article
• ISSN: 0022-4790
• DOI: 10.1002/(sici)1096-9098(199602)61:2<149::aid-jso11>3.0.co;2-4

✦ Synopsis

Two types of fragments of MAb A7 were produced to improve the efficacy and safety in targeting chemotherapy with neocarzinostatin. In this study, '2SI-labeled F(ab')z and Fab fragments of MAb A7 and 1251-labeled MAb A7 were injected intravenously into mice with pancreatic carcinoma xenografts, and the accumulation of each antibody in the tumors was compared. A greater amount of the I2'I-labeled Fab fragments of MAb A7 localized in the tumor 2 h following the injection than was observed with the other probes. Relatively less '251-labeled MAb A7 localized in the tumor 2 h following the injection than was observed with the other two probes. Moreover, reaction of rabbit antimouse IgG with the Fc portion, which is the most immunopotent region of the Fab and F(ab'), fragments of MAb A7 and MAb A7, was determined by ELISA; the weakest reaction was observed with the Fab fragments of MAb A7. These results suggest that the Fab fragments of MAb A7 may be more suitable carriers of an anticancer drug that is inactivated rapidly in the blood, such as NCS, in targeting chemotherapy than either intact MAb A7 or the F(ab'), fragments of MAb A7.