Antitumor effect of neocarzinostatin conjugated to human/mouse chimeric fab fragments of the monoclonal antibody A7 on human pancreatic carcinoma
✍ Scribed by Eigo Otsuji; Toshiharu Yamaguchi; Yoshihiro Yata; Hiroshi Nishi; Kazuma Okamoto; Katsunori Taniguchi; Makoto Kato; Tatsuya Kotani; Nobuki Yamaoka; Kazuya Kitamura; Toshio Takahashi
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 495 KB
- Volume
- 57
- Category
- Article
- ISSN
- 0022-4790
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✦ Synopsis
The anticancer agent neocarzinostatin (NCS) was bound covalently to human/mouse chimeric Fab fragments of the monoclonal antibody A7 to form the conjugate chA7Fab-NCS. The antitumor effect of chA7Fab-NCS was tested by measuring the inhibition of 3H-thymidine incorporation into human pancreatic carcinoma cells. The chA7Fab-NCS was approximately 2.3 times as effective as free NCS against human pancreatic carcinoma cells which reacted with the monoclonal antibody A7. The antitumor activity of chA7Fab-NCS was inhibited by excess chA7Fab. ChA7Fab-NCS had an antitumor effect equivalent to free NCS on human pancreatic carcinoma cells which did not react with the monoclonal antibody A7. ChA7Fab-NCS appears to be a potentially useful conjugate for immunotargeting chemotherapy against pancreatic carcinoma.