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Apparent Molecular Weight of Erythropoietin Determined by Gel Filtration

โœ Scribed by Olesen, H. ;Fogh, Jan

Book ID
114788021
Publisher
Wiley (Blackwell Publishing)
Year
2009
Weight
178 KB
Volume
5
Category
Article
ISSN
0036-553X

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Gel filtration studies on Sephadex G-75 demonstrate markedly increased elution volumes for proteins chromatographed with polyethylene glycol. As little as 5% (w/v) polyethylene glycol in the applied protein sample can reduce apparent molecular weight estimates by gel filtration as much as 55%. Furth