The Shandon Southern preparative polyacrylamide gel electrophoresis apparatus was modified to permit use with SDS (sodium dodecyl sulfate) containing systems. Improvements include the elimination of the troublesome lower gel and modification of the elution plate to prevent localized high current den
Apparatus for preparative separation of substances by disk electrophoresis in polyacrylamide gel
โ Scribed by A. I. Gusev
- Publisher
- Springer US
- Year
- 1969
- Tongue
- English
- Weight
- 475 KB
- Volume
- 68
- Category
- Article
- ISSN
- 0007-4888
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Various conditions were analyzed and optimized for the preparative elution of proteins from nitrocellulose membranes after transfer from sodium dodecyl sulfate (SDS)-polyacrylamide gels. The efficiency of elution was best using pyridine or acetonitrile elution solvents, intermediate for buffer conta
We are presenting a simple method for the quantitation of the radioactivity contained in individual bands of polyacrylamide or agarose gels following electrophoresis. Our technique essentially consists of localizing the individual bands by staining or another appropriate method, cutting out the indi