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Apoptosis in human lymphoblastoid cells induced by acivicin, a specific γ glutamyltransferase inhibitor

✍ Scribed by Riccardo Graber; Gabriele A. Losa

Publisher
John Wiley and Sons
Year
1995
Tongue
French
Weight
726 KB
Volume
62
Category
Article
ISSN
0020-7136

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✦ Synopsis

We examined the effects of acivicin, a specific inhibitor of the ectoenzyme y-glutamyltransferase (y-GT), on y-GT activity and apoptosis in 2 human T-lymphoblastoid CEM cell lines, CCRF and VBL-100. In both cell lines, acivicin was found to cause morphological and biochemical changes of apoptosis in a dosedependent manner. There was a close correlation between inhibition of y-GT activity and the emergence of apoptotic cells. However, VBL-I00 cells had a 50% higher y-GT basal activity than CCRF-CEM cells, their enzyme activity was more inhibited, and, they had a greater apoptotic response to acivicin. The y-GT-specific activity in apoptotiddead cells was also almost totally inhibited, while that of cells that remained alive after 5 days of acivicin treatment was not. These findings confirm that y-GT is implicated in the process of apoptosis of CEM cells.

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