๐”– Bobbio Scriptorium
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Apolipoprotein e genotyping methods for the clinical laboratory

โœ Scribed by Baku Maekawa; Thomas G. Cole; Richard L. Seip; David Bylund

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
856 KB
Volume
9
Category
Article
ISSN
0887-8013

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โœฆ Synopsis

mined by the three common alleles ~2 , ~3

and ~4 , we compared the radiolabeled allele-specific oligonucleotide (ASO) probe assay and the nonisotopic restriction isotyping assay. Leukocytic DNA was extracted from the blood of 93 patients after which the region containing two mutation points coding amino acid residues 11 2 and 158 was amplified by using the polymerase chain reaction (PCR).Amplified DNAfragments were spotted on nylon membranes, then hybridized for the AS0 probe assay. The amplified DNA fragments were also digested with re-

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Color Multiplexing Hybridization Probes Using the Apolipoprotein E Locus as a Model System for Genotyping
โœ Philip S. Bernard; Gregory H. Pritham; Carl T. Wittwer ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 140 KB

Fluorescent hybridization probes were multiplexed for color genotyping of the apolipoprotein E locus using model oligonucleotide targets. Fluorescence resonance energy transfer was observed during adjacent hybridization of 3-fluorescein-labeled "donor" probes paired with 5-labeled "acceptor" probes