Antimutagenic and promutagenic activity of ascorbic acid during oxidative stress

✍ Scribed by Gautam N. Bijur; Maria E. Ariza; Charles L. Hitchcock; Marshall V. Williams

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 104 KB
• Volume: 30
• Category: Article
• ISSN: 0893-6692
• DOI: 10.1002/(sici)1098-2280(1997)30:3<339::aid-em13>3.0.co;2-e

✦ Synopsis

Ascorbic acid (AA) has both antioxidant and proox-results in a statistically significant increase in both idant activities. However, there have not been any the cytotoxic and mutagenic effects of oxidative studies to elucidate the molecular mechanisms that stress when compared to cell populations exposed determine whether AA functions as an anti-or a only to the RGS. The results, using a novel histoprooxidant during oxidative stress. The results of chemical-computer image analysis system to detect this study, using the Chinese hamster ovary cell line hydrogen peroxide (H 2 O 2 ), also demonstrate that AS52 as a model system, demonstrate that there there is a direct correlation between the ability of is a temporal relationship between the anti-and AA to decrease the levels of H 2 O 2 in cells and the prooxidant activities of a physiologically relevant cytotoxic and mutagenic effects of oxidative stress. concentration of AA (50 mM) and oxidative stress. This study suggests that the time at which AA is Treatment of cells with AA (50 mM) 24 hr prior to administered in relation to exposure to oxidative treatment of the cells with a radical generating sys-stress has an impact on AA antimutagenic activity, tem (RGS) results in a statistically significant inhibi-and this may explain the conflicting results concerntion of the cytotoxicity and mutagenicity associated ing the effectiveness of AA as a cancer chemoprewith exposure of AS52 cells to oxidative stress. Con-ventive agent.