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Antibody-dependent cellular cytotoxicity against tumor cells. II. The promonocyte identified as effector cell

✍ Scribed by Wolfgang Domzig; Marie-Luise Lohmann-Matthes

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
619 KB
Volume
9
Category
Article
ISSN
0014-2980

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✦ Synopsis

Abstract

Macrophage precursor cells were cultured from the bone marrow of mice in a liquid culture system in the presence of conditioned medium. To separate their different maturation stages, they were passed through a discontinuous Ficoll density gradient, treated with iron plus magnet or passed through glass bead columns. The different maturation stages have been tested for their function in antibody‐dependent cellular cytotoxicity (ADCC) against tumor target cells and in lymphokine‐induced macrophage‐mediated cytotoxicity. It is shown that the promonocyte, a nonadherent, nonphagocytic precursor cell, is a highly potent cytotoxic effector cell against antibody‐coated tumor targets but is totally inactive as an effector cell in lymphokine‐induced macrophage‐mediated cytotoxicity. In contrast, in the lymphokine‐induced macrophage‐mediated cytotoxicity the cytotoxic effector cell is a mature macrophage. Thus, ADCC seems to be a function of the macrophage precursor promonocytes, whereas lymphokine‐induced cytotoxicity is performed by mature macrophages. The relationship of promonocytes and killer (K) cells in ADCC against tumor targets is discussed.

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