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Antibody affinity purification using metallic nickel particles

✍ Scribed by Jun Gao; Zhijun Li; Thomas Russell; Zhiyu Li

Book ID
113687706
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
692 KB
Volume
895-896
Category
Article
ISSN
1873-376X

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An affinity purification technique has been developed using mild elution conditions for the isolation of humanized murine and murine \(\mathrm{IgG}_{1}\). This technique is based on the innate affinity of \(\mathrm{IgG}_{1}\) for metal and utilizes immobilized metal-affinity chromatography. Antibody