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Antibodies against dehydration products of 15-keto-13,14-dihydro-prostaglandin E2

✍ Scribed by B.M. Peskar; B. Günter; Ch. Steffens; E.E. Kröner; B.A. Peskar

Book ID: 115911186
Publisher: Elsevier Science
Year: 1980
Tongue: English
Weight: 439 KB
Volume: 115
Category: Article
ISSN: 0014-5793
DOI: 10.1016/0014-5793(80)80740-x

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The main metabolite of prostaglandin E, (PGE2) 13,14-dihydro-Sketo-PGE, readily dehydrates and can subsequently form a cyclic derivative. This problem can be overcome by the immediate formation of oximes of the 9 and 15 ketones in aqueous solution followed by subsequent extraction, methylation and t