𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Antibiotic-resistance cassettes for Bacillus subtilis

✍ Scribed by Anne-Marie Guérout-Fleury; Kamran Shazand; Niels Frandsen; Patrick Stragier

Book ID
116133194
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
124 KB
Volume
167
Category
Article
ISSN
0378-1119

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Among spontaneously occurring antibiotic-resistant mutants of Bacillus subtilis 168 we have identified a sub-class that is conditionally sporulative. Mutants in this sub-class are resistant to antibiotic during vegetative growth but are sensitive during sporulation. Mutants conditionally-resistant t

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✍ Kreft, Jürgen ;Burger, Klaus J. ;Goebel, Werner 📂 Article 📅 1983 🏛 Springer 🌐 English ⚖ 689 KB

Bifunctional recombinant plasmids were constructed, comprised of the E. coli vectors pBR322, pBR325 and pACYC184 and different plasmids from Gram-positive bacteria, e.g. pBSU161-1 of B. subtilis and pUB110 and pC221 of S. aureus. The beta-lactamase (bla) gene and the chloramphenicol acetyltransferas