Anti-tumor effect of internal image bearing anti-idiotypic monoclonal antibody in relation to GM3 ganglioside

✍ Scribed by Tsuneharu Jinnohara; Masayuki Tsujisaki; Shigeru Sasaki; Yuji Hinoda; Masaru Taniguchi; Kohzoh Imai

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: French
• Weight: 412 KB
• Volume: 76
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/(sici)1097-0215(19980504)76:3<345::aid-ijc11>3.0.co;2-a

✦ Synopsis

Anti

-idiotypic antibodies are a new type of useful tools for the possible treatment of cancer patients, since some act as antigen specific immunomodulators. Anti-idiotypic monoclonal antibody (anti-Id MAb) D704 (Ab2) was established which bore the internal image of the determinant defined by MAb M2590 (Ab1) against a sialic acid residue on GM 3 ganglioside. In an in vivo syngeneic tumor system, anti-Id MAb D704 was more effective in preventing tumor progression, as compared with anti-GM 3 MAb or no treatment. Significant suppression of tumor growth and prolongation of survival by administration of anti-Id MAb D704 in an animal group inoculated with 1 ؋ 10 4 /mouse melanoma cells were seen, but not in a group inoculated with 5 ؋ 10 4 cells/mouse. In an active specific immunotherapy protocol utilizing Ab2, the activity of antianti-Id antibodies (Ab3) specific for GM 3 (antigen) which has a weak immunogenicity only, was maintained for more than 3 months. Ab2 generated cellular anti-tumor immune responses, including delayed type hypersensitivity (DTH) reaction. Immunohistological analysis indicated a marked infiltration of CD4 and CD8 positive cells into the DTH sites. Our results suggest that internal image bearing anti-Id MAbs have a therapeutic potential against tumors if the number of melanoma cells is relatively low or if hosts are at an early stage of melanoma progression.