Anti-idiotypes against anti-H−2 antibodies VI. Detection of shared idiotypes among monoclonal anti-H−2 antibodies

✍ Scribed by Jeffrey A. Bluestone; Hugh Auchincloss Jr.; David H. Sachs; Matthias Fibi; Günter J. Hämmerling

• Publisher: John Wiley and Sons
• Year: 1983
• Tongue: English
• Weight: 710 KB
• Volume: 13
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830130611

✦ Synopsis

Anti-idiotypes produced against monoclonal anti-H-2 antibodies have been used to examine idiotope sharing among a panel of anti-major histocompatibility complex (MHC) antibodies detecting the same and different specificities. Both xenogeneic anti-100-30 and anti-3-83, which did not react with predominant idiotypes in conventional alloantisera, detected idiotopes on 3-83 and 100-30 as well as on 2 other monoclonal anti-H-2Kk antibodies. All 4 monoclonal antibodies recognized the same epitope cluster on the Kk molecule and detected the same serological specificity. H-2.5. In contrast, syngeneic and allogeneic anti-idiotype produced against the same monoclonal antibodies did not detect these cross-reactive determinants. In several instances, xenogeneic anti-idiotype reacted with anti-MHC antibodies which recognized distinct H-2 determinants, suggesting that anti-MHC antibodies detecting the same or different specificities may share idiotypic determinants. These reagents may be useful probes of the anti-MHC immune repertoire.