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Anisotropic rotational diffusion of indole in cyclohexane studied by 2 GHz frequency-domain fluorometry

✍ Scribed by Joseph R. Lakowicz; Ignacy Gryczynski; Wieslaw M. Wiczk

Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 554 KB
Volume: 149
Category: Article
ISSN: 0009-2614
DOI: 10.1016/0009-2614(88)87209-9

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✦ Synopsis

We measured the frequency response of the polarized emission of non-quenched and quenched indole in cyclohexane at 2O"C, up to 2 GHz. Non-radiative energy transfer from indole to 1-stilbene was used to decrease indole decay time. Quenching increases the fraction of the total emission, which occurs on a picosecond timescale, and thereby provides increased information on rapid rotational motions. The measurement of quenched sample allowed resolution of the anisotropic rotation of indole with correlation times of 17 and 73 ps. The longer correlation time is consistent with that expected for a sphere with stick boundary conditions, the shorter with the slip boundary condition for an oblate ellipsoid.

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