Enhanced resolution of anisotropic rotational diffusion by multi-wavelength frequency-domain fluorometry and global analysis

✍ Scribed by Ignacy Gryczynski; Henryk Cherek; Gabor Laczko; Joseph R. Lakowicz

• Publisher: Elsevier Science
• Year: 1987
• Tongue: English
• Weight: 606 KB
• Volume: 135
• Category: Article
• ISSN: 0009-2614
• DOI: 10.1016/0009-2614(87)85141-2

✦ Synopsis

Frequency-dependent differential polarized phase and modulation data were used to measure time-dependent anisotropy decays of 9-aminoacridine and Y-base. The data were obtained at various excitation wavelengths, at which the fundamental anisotropies (ra) varied from -0.17 to 0.373. The different values of r0 result in different contributions of the multiple correlation times to the anisotropy decay. The multi-wavelength data were analyzed globally, revealing both molecules to be anisotropic rotators. The rotational motions of Y-base are more anisotropic than those of 9-aminoacridine. In propylene glycol at -5°C the two correlation times for Y-base were found to be 2.1 and 12.1 ns. For 9aminoacridine the correlation times were 9.5 and 14.1 ns.