The components of a 99mTc(Sn)-EHDP complex mixture were separated by means of normal pressure and high-pressure anion exchange chromatography, on DEAE-Trisacryl and Aminex, respectively. Precautions were taken to prevent the dissociation of the complexes during chromatography. The charges of the com
โฆ LIBER โฆ
Anion exchange chromatography of 99mTc(Sn)-MDP complexes: Influence of eluent composition, determination of void volume and charge of the main component
โ Scribed by Y.M. Huigen; T.G. Tji; W.J. Gelsema; C.L. de Ligny
- Publisher
- Elsevier Science
- Year
- 1988
- Weight
- 476 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0883-2889
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The binding of 99mTc(Sn)-MDP to human serum albumin and other blood proteins was investigated by gel chromatography and ultrafiltration. During gel chromatography dissociation of the 99mTc(Sn)-MDP-protein complex occurs: thus, it is not a suitable technique for the determination of protein binding.