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Anhydrobiosis in nematodes: Biosynthesis of trehalose

✍ Scribed by Loomis, Stephen H. ;Madin, K. A. C. ;Crowe, John H.

Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
560 KB
Volume
211
Category
Article
ISSN
0022-104X

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✦ Synopsis

Abstract

The pathway for the synthesis of trehalose in Aphelenchus avenae was demonstrated. As in other systems, the enzymes involved in the synthesis of trehalose of A. avenae were found to be trehalose phosphate synthetase (TPS) and trehalosephosphate phosphatase (TPP). Some of the characteristics of trehalose phosphate synthetase were studied. It was found that the rate limiting step in the reaction is catalyzed by TPS. Partially purified TPS was specific for UDPG as the glucosyl donor, and showed a pH optimum of 8.0. The partially purified enzyme has a Km of 0.6 mM and a V~max~ of 2.2 Γ— 10^βˆ’3^ ΞΌmoles/mg protein min for glucose‐6‐phosphate, and a Km of 2.4 mM and V~max~ of 2.5 Γ— 10^βˆ’3^ ΞΌmoles/mg protein minfor UDPG.

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