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Angiotensin II type 2 receptor gene polymorphisms and cardioprotective role in essential hypertension

✍ Scribed by Min Zhang; Hong Ma; Bo-song Wang; Yong-zhong Zhao

Publisher: Springer
Year: 2006
Tongue: English
Weight: 328 KB
Volume: 21
Category: Article
ISSN: 0910-8327
DOI: 10.1007/s00380-005-0865-1

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## Detection conditions: Forward primer: 5´-ATT ACG TCC CAG CGT CTG AG-3Ŕ everse primer: 5´-ATA AAT CAG CTT GCT TAG TGC C-33 5 cycles; denaturation at 94°C (20 s), annealing at 60°C (20 s), extension at 72°C (20 s), final extension 72°C (10 min) 10% PAA gels (49:1, acrylamide:bisacrylamide), 0.5XTB