A rapid bioluminometric technique for real-time detection of known single-base changes is presented. The concept relies on the measurement of the difference in primer extension efficiency by a DNA polymerase of a matched over a mismatched 3' terminal. The rate of the DNA polymerase-catalyzed primer
β¦ LIBER β¦
Analytical Assays Based on Detecting Conformational Changes of Single Molecules
β Scribed by Giovanni Zocchi
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 275 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1439-4235
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## Abstract A powerful combination of molecular beacon and luminescence resonance energy transfer technology reveals alterations in nucleic acid structure by as little as a single nucleotide in a novel hybridization proximity assay. The assay measures the length of a singleβstranded target when a t