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Use of conformation sensitive gel electrophoresis to detect single-base changes in the gene for COL10A1

✍ Scribed by Dr. Jarmo Körkkö; Jeffrey Milunsky; Darwin J. Prockop; Leena Ala-Kokko


Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
243 KB
Volume
11
Category
Article
ISSN
1059-7794

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Gilbert's syndrome, due to reduced hepatic bilirubin glucuronidation is associated with the presence of two extra nucleotides (TA) in the promoter region of the UDP-glucuronosyltransferase 1 (UGT1A1) gene. A rapid method was developed to detect this genetic polymorphism, using double gradient denatu