Analytical and micropreparative ultrahigh resolution of oligonucleotides by polyacrylamide gel high-performance capillary electrophoresis
β Scribed by Guttman, A.; Cohen, A. S.; Heiger, D. N.; Karger, Barry L.
- Book ID
- 120051336
- Publisher
- American Chemical Society
- Year
- 1990
- Tongue
- English
- Weight
- 648 KB
- Volume
- 62
- Category
- Article
- ISSN
- 0003-2700
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High-performance capillary sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) has been developed for the separation and molecular weight (MW) determination of peptides and proteins. In this work, acrylamide was polymerized in fused-silica capillaries of 75 microns I.D. and 10 or 20
## Abstract By employing linear polyacrylamide (LPA) as a sieving matrix, oligonucleotides and DNA sequencing fragments have been separated by capillary electrophoresis. A commercially available apparatus equipped with a laserβinduced fluorescence (LIF) detection system has been utilized, but the c