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Analysis of yeast protein kinases using protein chips

✍ Scribed by Snyder, Michael; Zhu, Heng; Klemic, James F.; Chang, Swan; Bertone, Paul; Casamayor, Antonio; Klemic, Kathryn G.; Smith, David; Gerstein, Mark; Reed, Mark A.


Book ID
109829431
Publisher
Nature Publishing Group
Year
2000
Tongue
English
Weight
879 KB
Volume
26
Category
Article
ISSN
1061-4036

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Multiforms of megamodulin-dependent protein kinases (M-PK) were partially purified from baker's yeast by excluding endogenous megamodulin with histone, and then by gel filtration with Sephadex G-200. The stimulation of M-PK in the presence of Mg 2+, Mn 2+ or Co 2+ was enhanced by yeast megamodulin.