Multiforms of megamodulin-dependent protein kinases from baker's yeast

✍ Scribed by Wu-Nan Kuo

• Book ID: 104769031
• Publisher: Springer
• Year: 1984
• Tongue: English
• Weight: 267 KB
• Volume: 64
• Category: Article
• ISSN: 0300-8177
• DOI: 10.1007/bf00420926

✦ Synopsis

Multiforms of megamodulin-dependent protein kinases (M-PK) were partially purified from baker's yeast by excluding endogenous megamodulin with histone, and then by gel filtration with Sephadex G-200. The stimulation of M-PK in the presence of Mg 2+, Mn 2+ or Co 2+ was enhanced by yeast megamodulin. In addition, similar augmented activity of M-PK was also noted in the presence of Mg 2+ by megamodulins prepared from E. coli, bovine brain and wheat germ.