Thirty surgical samples of squamous cell carcinoma of the cervix obtained from Chinese women were analysed for the presence of human papillomavirus (HPV) types 16 and 18 using Southern blot hybridization procedure. HPV16 was detected in 53% while HPV18 was found in only 6% of the samples analyzed. W
Analysis of the unoccupied site of an integrated human papillomavirus 16 sequence in a cervical carcinoma
β Scribed by Kong-Bung Choo; Hsien-Hsiung Lee; Lip-Nyin Liew; Kowit-Yu Chong; Huar-Fen Chou
- Book ID
- 113209300
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 767 KB
- Volume
- 178
- Category
- Article
- ISSN
- 0042-6822
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## Abstract A protocol for a rapid physical mapping of the integrated type 16 human papillomavirus (HPV16) sequences in biospied and paraffinβembedded archival cervical cancer samples is described. The procedure involves the use of an anchor primer and a mixture of indicator primers in a multiplex
A total of six cervical carcinoma cell lines, of which two contained human papillomavirus (HPV) type 16 and four contained HPV type 18 DNA, were examined for viral RNA expression by spot-blot hybridization. All six cell lines contained HPV-specific cytoplasmic poly(A)-containing RNA. The same RNA sp