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Analysis of the site of action of the amdR product for regulation of the amdS gene of Aspergillus nidulans

✍ Scribed by Littlejohn, Tim G. ;Hynes, Michael J.

Publisher
Springer
Year
1992
Tongue
English
Weight
816 KB
Volume
235
Category
Article
ISSN
0026-8925

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✦ Synopsis

The amdR gene of Aspergillus nidulans regulates a number of structural genes in response to omega amino acid inducers. The site of action of the amdR product on expression of the amdS gene was investigated by studying the effects of changes in the 5' region of amdS, generated in vitro, on the induction, and on responses of an amdS-lacZ fusion gene to an amdRc allele. A sequence was identified that is sufficient for amdR regulation and that shows identity with sequences involved in amdR regulation of the gatA and lam genes. This sequence includes a CCAAT sequence and it was shown that this sequence is an important element in setting the basal level of amdS expression.

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