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Analysis of the sex chromosome constitution of single bovine spermatozoa by polymerase chain reaction (PCR)

✍ Scribed by Klaus I. Matthaei; Charles M. Herr; Mark P. Bradley; Ken C. Reed


Book ID
118378779
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
89 KB
Volume
33
Category
Article
ISSN
0093-691X

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The polymorphic exon 2–exon 3 region of bovine major histocompatibility complex (MHC) class I genes was amplified by polymerase chain reaction (PCR) from genomic DNA samples with characterized class I polymorphism. The primers for amplification were designed in conserved regions at the borders of ex