Polymorphism of bovine major histocompatibility complex (MHC) class I genes revealed by polymerase chain reaction (PCR) and restriction enzyme analysis
✍ Scribed by S. M. S. Sawhney; D. W. Taylor; G. C. Russell
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 151 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0268-9146
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✦ Synopsis
The polymorphic exon 2–exon 3 region of bovine major histocompatibility complex (MHC) class I genes was amplified by polymerase chain reaction (PCR) from genomic DNA samples with characterized class I polymorphism. The primers for amplification were designed in conserved regions at the borders of exons 2 and 3, based on all available cDNA sequences. The primers should, therefore, amplify most expressed class I genes, but may also amplify non‐expressed class I genes. The PCR amplified class I gene fragments of 700 bp were characterized on the basis of restriction fragment length polymorphism (RFLP). The PCR–RFLP analysis of class I genes showed that the bands in each digestion could be classified as non‐polymorphic, as shared between several bovine lymphocyte antigen (BoLA)‐A types, or as specific to a single BoLA‐A type. The same primers were then used for amplification of class I gene fragments from eight Sahiwal animals, a breed which originated in the Indian subcontinent. These studies showed that BoLA class I PCR–RFLP could be used to study class I polymorphism in family groups.