Analysis of the polysaccharides of some soil bacteria by gas chromatography

✍ Scribed by M. Wurst; V. Vančura; L. Kalachová

• Publisher: Elsevier Science
• Year: 1974
• Tongue: English
• Weight: 474 KB
• Volume: 91
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)97927-0

✦ Synopsis

A method For the determination of the composition of the extracellular polysaccharides of some soil bacteria by gas chromatography was worked out, Monosaccharides originating from the hydrolysis of polysaccharides were separated as trimethylsilyl derivatives. In addition to the main components, cc-and P-D-glucose, D-galactose and D-mannose, other hexoses and pentoses, D-fructose, 6-deoxy-L-mannose, D-ribose, D-xylose and uranic acids, i.e. D-glucuronic, D-gahCtUrOniC and Dmannuronic acids, could be detected in the extracellular polysaccharide. Composition of the extracellular polysaccharide was determined in several species of soil bacteria, including Achromobacter deficatulum, Pseudomonas dcsmolyticum, Xanthomonas phaseoli var. fuscans and Azotobacter chroococcum.