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Analysis of the Neurotoxin β-ODAP and Its α-Isomer by Precolumn Derivatization with Phenylisothiocyanate

✍ Scribed by J.K. Khan; N. Kebede; Y.H. Kuo; F. Lambein; A. Debruyn

Book ID: 102560420
Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 274 KB
Volume: 208
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.1993.1038

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✦ Synopsis

A method is presented for determining (3-N)-oxalyl2,3-diaminopropanoic acid ( (\beta)-ODAP), the most potent neurotoxic substance of the seeds and seedlings of Lathyrus sativus, and its much less toxic 2 -isomer ( (\alpha) ODAP). The separation of the two forms is achieved after derivatization with phenylisothiocyanate employing a HPLC system. This method was used to monitor the isomerization of (\beta)-ODAP to (\alpha)-ODAP at different time intervals and to quantify the toxin level in seed extracts. cे 1993 Academic Press, Inc.

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