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Analysis of the glutathione conjugate of paracetamol in human liver microsomal fraction by liquid chromatography mass spectrometry

✍ Scribed by Natalie J. Thatcher; Stephen Murray

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
80 KB
Volume
15
Category
Article
ISSN
0269-3879

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✦ Synopsis

Abstract

An assay based on microbore liquid chromatography–positive ion electrospray mass spectrometry with selected ion recording has been developed for the measurement of the glutathione conjugate of paracetamol (acetaminophen) in human hepatic microsomal incubations. A deuterated analogue of the glutathione conjugate was synthesized for use as an internal standard and extraction was carried out on Oasis™ solid phase extraction columns. The limit of quantification of the glutathione conjugate was 10 ng/incubation and the intra‐assay coefficient of variation at this concentration was 6.8%. Copyright © 2001 John Wiley & Sons, Ltd.

Abbreviations used:

ESI

electrosprayionization

NABQI

N‐acetyl‐p‐benzoquinoneimine

SIR

selected ion recording

SRM

selected reaction monitoring.

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