Analysis of the gel electrophoresis of looped protein-DNA complexes by computer simulation
β Scribed by John R. Cann
- Book ID
- 119443731
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 897 KB
- Volume
- 216
- Category
- Article
- ISSN
- 0022-2836
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## Factors that affect the stability of protein-DNA complexes during gel electrophoresis The gel electrophoresis mobility shift assay is widely used for qualitative and quantitative characterization of protein complexes with nucleic acids. Often it is found that complexes persist within electropho
A method is described to determine the presence and the relative amount of proteins within specific protein-DNA complexes. The system studied is the LexA repressor from Escherichia coli and its interaction with the operator of the caa gene encoding the bacterial toxin colicin A. After separation of